INVESTIGADORES
MONESTEROLO Noelia Edith
congresos y reuniones científicas
Título:
EFFECT OF GLUCOSE ON DISTRIBUTION AND POSTRANSLATIONAL MODIFICATIONS OF TUBULIN IN ERYTHROCYTES
Autor/es:
NIGRA AD; AMAIDEN MR; PERETTI SOM; RIVELLI JF; MONESTEROLO NE; CASALE CH; SANTANDER VS
Lugar:
Mendoza
Reunión:
Congreso; XLVIII Reunión Anual de la Sociedad Argentina de Investigación Bioquímica y Biología Molecular; 2012
Institución organizadora:
Sociedad Argentina de Investigación Bioquímica y Biología Molecular
Resumen:
In this work distribution of different isotypes
of tubulin in erythrocytes treated with high glucose concentrations was analyzed.
Results shown that, in human erythrocytes, glucose induce translocation of
tubulin from sedimentable fraction to the membrane. Similar results are
observed in Wistar rats injected with high glucose concentrations; in this case
the maximum levels of tubulin in the membrane was reached during the peak of
glycemia after the treatment. When the glycemia values return to his basal
values, tubulin is relocated in the sedimentable fraction. Analysis of
different tubulin isotypes in the membrane showed an increase of both detyrosinated
and acetylated tubulin, whereas the tyrosinated tubulin is diminishes in this
fraction. These results would explain the fact that in diabetic subjects NKA
activity is decreased. On the other hand, analysis of tubulin content in
sedimentable fraction in erythrocytes treated with nocodazol showed that
tubulin is lost when cells are treated with glucose. These results suggest that
microtubules are present in the sedimentable fraction and they are more
dynamics in erythrocytes treated with glucose. In conclusion, glucose induces
in mammals? erythrocytes a redistribution of tubulin and changes in the
different isotypes, fact that might be related to the inhibition of the NKA activity.