INVESTIGADORES
LORENZO PISARELLO Maria Jose
congresos y reuniones científicas
Título:
Characterization of intestinal microbiota of children and isolation of lactobacilli with probiotic potential
Autor/es:
LORENZO PISARELLO, MJ; VINTIÑI, EO; PAGANI, F; GONZALEZ, S; MEDINA, M
Lugar:
Tafi del Valle
Reunión:
Jornada; XVIII JornadasCientificas - Asociación de Biología de Tucumán; 2011
Institución organizadora:
Asociación de Biología de Tucumán
Resumen:
The intestinal microbiota (IM) is a complex
microenvironment and includes a variety of bacterial genera and species among
which the Lactobacilli are one of the main genera used as
probiotics.Objective:To characterize the major bacterial genera present in the
IM of eutrophic children and isolate strains of Lactobacillus for potential use
as probiotics in human health. Stool of 15 healthy children were analyzed.MM:1)Count
by conventional techniques: total aerobes and anaerobes,Enterobacteriaceae and
lactobacilli.The N° of bacteria was determined by plate count and the results
were expressed as CFU/g of feces, 2) Isolation of lactobacilli and evaluation of
their morphology (GRAM stain), specific biochemical properties (API system),
resistance to acid pH and bile. The results are expressed as mean total counts
x g of faeces: Anaerobes: 2x109 CFU/g,Aerobes: 5x109
CFU/g, Enterobacteriaceae: 2x106 CFU /g and Lactobacillus: 3x106
CFU/g. It were isolated 2 strains of lactobacilli that with high resistance to
acid pH and bile, fundamental requirements of a strain, to be defined as a
probiotic. Further studies are necessary to their potential use in the
development of products for preventive applications and/or particular
therapeutic