Surfactant type influence on the catalytic activity of soybean Lipoxygenase

MEDINA, A.V.; CHAILLOU, L.L.; NAZARENO M.A.

Córdoba

Congreso; XLII Reunión Anual de la Sociedad Argentina de Biofísica. SAB 2013; 2013

SAB

The catalytic activity of enzymes is influenced by many physical and chemical factors. Model systems are used to study this activity based on the properties of amphiphilic molecules to self-organize in solution, such as micellar systems [1]. Lipoxygenases (LOX) are dioxygenases which catalyze the initial reaction of oxidation of polyunsaturated fatty acids, containing a 1,4-cis,cis-pentadiene system yielding primary products with conjugated diene hydroperoxides [2]. In food, these enzymes play an important role in oxidative processes, therefore several technological processes have been developed for the purpose of eliminating or reducing its activity [3-4]. The surfactant nature of microemulsions has also influence in the reactivity of diverse enzymes such as LOX. Main objectives were to study the influence of different type of surfactants on the soybean LOX catalytic activity and to determine the kinetic parameters associated with the oxidation reaction.LOX induces an enhancement in surface pressure that can be related to electrostatic or non-specific interaction.The chemical reaction produced in DSPC:LA is observed by a non linear diffsuion pattern and an insertion of zeta potential in contrast to control assays with DSPC:SA membranes