Estudio in vitro de la citotoxicidad de nanopartículas de TiO2

JONATHAN M. SCHUSTER; MARGARITA E. LACZESKI; MARIO R. ROSENBERGER; CARLOS E. SCHVEZOV

Santa Fé

Congreso; Congreso Internacional De Metalurgia y Materiales. Sam ? Conamet / Iberomat / Materia 2014; 2014

sam - Asociación Argentina de Materiales

Titanium dioxide (TiO2) coatings are widely employed in human implants due to their good corrosion resistance and tissue biocompatibility. In the case of moving implants the TiO2 coating is subject to wear releasing nano and micrometrics debris to the surrounding system. The debris may promote or produce redox reactions due to its electronic properties acting as donor or acceptors of electrons in its interaction with the many surrounding organic compounds, this may produce the formation of free radicals which are strongly oxidant degrading the organic compounds. When the debris are of nanometric size the surface /volume ratio is larger and therefore the relative activity per unit of mass, and the consequent relative higher degree of oxidative stress of the cells. In the present paper the capacity of TiO2 nanoparticles (Degussa P-25) to produce cell damage or death in a short period of time (3 hours) is evaluated and reported. The cells studied were the lymphocytes and the following techniques to determine the effects on the cells were used; trypan blue exclusion test, DNA laddering and observation of apoptotic bodies. The results obtained with each technique show that the nanoparticles of TiO2 produce damage in the cell membrane of the isolated lymphocytes, no apoptotic bodies were observed in the treatments with the nanoparticles and in the case of the DNA laddering technique no fragmentation of the DNA (apoptosis marker) was detected on the cells.