Use of Chorella kessleri extracts for the protection of hepatocyte primary cultures from pig and human livers

ITHURALDE E,; LORENTI A,; ROGATI S,; BARBICH M,; CHAUFAN G,; JUÁREZ A,; RÍOS DE MOLINA MC.

Pinamar, Buenos Aires, Argentina

Congreso; XLI Reunión Anual de la SAIB y Biología Molecular; 2005

SAIB

The antioxidant capability of extracts from C. kessleri was assayed in this work. Primary cultures of pig hepatocytes were protected during ?ex vivo? oxidative stress processes. This protection reached 100 % even when the lowest concentrations of extracts were tested, against both endogenous and exogenous oxidant agents. Preliminary results with human hepatocytes cultures (1 month cultures) showed different morphologic patterns with the different concentrations of the extracts used (from 1:4000 to 1:20000). Extraction and characterization of active principles were carried out, in order to scream the sub-cellular localization. The highest antioxidant activity was found on the particulate fraction. The UV/visible spectrum analysis from aqueous and methanol extracts showed the presence of different substances, some of them being characteristic algae pigments. These results show that the antioxidant compound (present in great amounts in C. kessleri obtained by this methodology allow us to protect hepatocyte primary cultures.