INVESTIGADORES
ZAVALA Jorge Alberto
congresos y reuniones científicas
Título:
Southern green stink bug (Nezara viridula) attack alters soybean gene expression related with cell wall proteins in developing seeds.
Autor/es:
ILINA, N.; BARNETO, J; ZAVALA, J. A.
Lugar:
Urbana
Reunión:
Encuentro; Joint meeting of the International Society for Chemical Ecology and the Chemical Signals in Vertebrates group.; 2014
Resumen:
Southern
green stink bug (Nezara viridula) attack alters soybean gene expression related
with cell
wall proteins in developing seeds
Natalia Ilina, Jesica A. Barneto, and Jorge A. Zavala, INBA
CONICET
Presenter:
Jorge A. Zavala, INBA CONICET
Plant cell
walls are complex and dynamic structures with several functions, mainly being
a physical
barrier against pathogens and herbivores. Southern green stink bug (Nezara
viridula)
is one of the most important pests of soybean (Glycine max) in South America.
Plants are
constantly challenged by insect attack, thus they have evolved an array of
defense
mechanisms
against herbivores. Cell wall can be an important barrier against insects, such
as stink
bugs. However seeds cell wall-insect interaction is an unexplored field. Here
we
studied
gene expression of cell wall associated proteins involved in the modification
of
the cell
wall soybean seeds. We proposed that the stink bug attack cause a rearrangement
of the cell
wall. These changes may be regulated by hormones related to plant defenses
such as
jasmonic acid (JA) and salicylic acid (SA). To address this question we apply
to
developing
seeds JA or SA, allowed stink bugs to feed on seed and analyzed the cell wall
transcriptional
response. We found that 72h after stink bugs attack increased expansine
(EXP), xyloglucan endo-transferase (EXT), pectate lyase (PL)
y polygalacturonase (PG)
expression.
Interestingly, when SA was applied we detected a strong induction for PG,
PL,
xyloglucan fucosyltransferase (XFT) and xyloglucan endo-transglycosylase (XET)
transcripts;
while with JA treatment only EXP and XFT were up-regulated. Our results
suggest
that stink bug attack increases expression of cell wall genes transcription
that
produce
chemical rearrangements regulated by both JA and SA.