INVESTIGADORES
ROSSO Silvana Beatriz
congresos y reuniones científicas
Título:
Role of Wnt5a in the neuronal development and its participation on the glyphosate induced neurotoxicity
Autor/es:
COULLERY, ROMINA; ROSSO SB
Lugar:
Huerta Grande
Reunión:
Congreso; XIX Congreso Anual de la Sociedad Argentina de Investigaciones en Neurociencia; 2014
Institución organizadora:
Sociedad Argentina de Investigación en Neurociencia.
Resumen:
Neuronal cells must develop and acquired its characteristic polarized morphology in order to establish complex neuronal networks. Among different molecules involved in neuronal development, the Wnt factors have been identified as key morphogens that regulate different neuronal events. Proper control of neuronal morphogenesis is critical and sensitive; therefore, it can be affected by environmental substances, resulting in an abnormal functionality of the nervous system. In this work, we study the Wnt5a effects during early stages of neuronal development. Our results showed that cultured hippocampal neurons stimulated by Wnt5a exhibited longer and more branched axons compared to controls. To go further we identified the Wnt pathway mediating this process. Our observations showed that Wnt5a positively regulated axon growth through the activation of CaMKII, an effector of the non-canonical Wnt/Ca pathway. On the other hand, we have previously found that cultured neurons are highly vulnerable to the exposition to glyphosate (the active component of Roundup).Neurons exposed to glyphosate exhibited a markedly delay in their development characterized by a decrease in axonal and dendrite growth and complexity. To identify the intracellular mechanism, we analyzed the Wnt5a/CaMKII pathway and observed a significantly reduction in the Wnt5a expression, and CaMKII activity. Taken together, we postulate that Wnt5a/CaMKII pathway as a main target pathway of glyphosate neurotoxicity.