Human papillomavirus DNA spectrum in oral lesions: condyloma acuminata, ameloblastomas and squamous cell carcinomas

GÓMEZ MA; ABBA MC; MICINQUEVICH S; CASARIEGO Z; GOLIJOW CD

Florianópolis, Brasil

Conferencia; 19th International Papillomavirus Conference; 2001

International Papillomavirus Society (IPS)

Background: Human Papillomavirus (HPV) infection is a sexual trasmited disease and is the cause of different pathologies related to oral and genital carcinomas. Condyloma acuminata are common pseudotumoral lesions that could been found in the oral tract. Intrabony Ameloblastomas are odontogenic tumors of ectodermal origin, usually located on the mandibular or maxillary gingiva where. According to other authors, HPV would not directly implicated in the patogenesis of this disease. Squamous cell carcinoma of the oral cavity involves different etiological factors and mechanisms. The association of HPV infection and squamous cell carcinoma is not as clear as that found in cervical cancer. The aim of present work was to study the prevalence and genotypes of HPV-DNAs in a serie of condiloma acuminata, squamous cell carcinomas and ameloblastomas from the oral tract. Material and Methods: Forty formalin-fixed, paraffin-embedded tissue blocks (15 oral Condylomas acuminata, 9 Ameloblastomas and 16oral Carcinomas) were evaluated for HPV presence by nested polymerase chain reaction using My 09/11 and Gp 05/06 primers. The viral genotype was analysed by means of the single-stranded conformation polymorphisms (SSCP) methodology. Results: All DNA samplesobtained from biopsies were satisfactory for PCR analysis. For condylomas, HPV-DNA was found in 86.67%. HPV-6/11 were the prevalent types, comprising 73.34% of the positive cases. All the ameloblastomas were positives for HPV-DNA. In this kind of lesions HPV-6/11 comprise 55.56% meanwhile 44.44% corresponded to HPV-16/18. For squamous cell carcinomas, the prevalence of HPV-DNA was 68.8 % and the most frecuently viral types was HPV-16 (53.8%), followed by HPV-6/11 (46.1%). Conclusions: The association between HPV infection and oral lesions was demostrated for the oral condylomas. Surprisingly, all the ameloblastomas were HPV positives, suggesting a role of the virus in the ethiology of the lesion. The viral prevalence found in cancerous lesions reinforces the concept of heterogenic nature of oral cancer. The findings indicate that HPV infection may be related to a proportion of squamous cell carcinomas, but its association is not as clear as that found in cervical cancer. Perhaps oral infection with HPV is a circumstance that increase the probability of malignancy. No preferential association has been demonstrated between specific HPV types and ameloblastoma. We conclude that the PCR method is a useful and reliable method for the detection of HPV DNA within oral mucosa cells.