LEAF ANTIFUNGALS OF SCHINOPSIS: ISOLATION, IDENTIFICATION AND BIOACTIVITY ON TOXIGENIC SPECIES OF FUSARIUM

ARISTIMUÑO FICOSECO, M.E.; SAMPIETRO, D.A.; VATTUONE, M.A.; CATALAN, C.A.N.

Tucuman

Congreso; XXX ANNUAL SCIENTIFIC MEETING TUCUMAN BIOLOGY ASSOCIATION; 2013

TUCUMAN BIOLOGY ASSOCIATION

The aim of this work was to isolate and identify antifungals from leaf extracts of Schinopsis lorentzii and S. haenkeana, useful for the control of etiological agents of ear rotting in maize and wheat. Leaves of Schinopsis (S. lorentzii, S. haenkeana) were sequentially extracted with dichloromethane and ethyl acetate. The extracts obtained were evaporated to dryness. The dry residues were suspended in 50 ml of methanol and filtered to obtain the methanolic fractions of dichloromethane (FmCH2CL2) and ethyl acetate extracts (FmAcOEt). The components of these fractions were separated by column chromatograpy (CC) in Silica gel and gas chromatography coupled to mass spectrometry. The antifungal activity was evaluated by bioautography and microdilution assays in liquid medium, and CI50 was determined. The separation in CC of components from FmCH2CL2 and FmAcOEt, and the bioautographic assay showed a band of low polarity (Rf= 0.7) with antifungal activity on Fusarium. The band contained alkylcatechols (ALQCt) and lupeol (LUP). The CI50 obtained were 32.23 g/ml (F. graminearum) and 150 g/ml (F. verticillioides) for ALQCt, and 75.87 g/ml (F. graminearum) and 810.7 g/ml (F. verticillioides) for LUP. F. graminearum was more sensitive to the identified antifungals than F. verticilloides. The lowest CI50 were obtained for ALQCt on both Fusarium species.