INVESTIGADORES
LOCATELLI Paola
congresos y reuniones científicas
Título:
Adipose stromal cells overexpressing human VEGF protect against ischemic muscle lesions in rabbits with experimental hindlimb ischemia
Autor/es:
OLEA FERNANDA DANIELA; LOCATELLI PAOLA; HNATIUK ANNA; YANNARELLI GUSTAVO; GIMÉNEZ CARLOS SEBASTIÁN; VALDIVIESO LEON; ROCHA ESTEFANÍA; LAGUENS RUBÉN; CROTTOGINI ALBERTO
Lugar:
Foz de Iguazú
Reunión:
Congreso; Congreso Panamericano de Fisiología; 2014
Institución organizadora:
Sociedade Brasileira de Fisiología
Resumen:
We have shown that plasmid-mediated human VEGF165 (pVEGF) gene transfer induces angio-arteriogenesis and affords protection against ischemic muscle lesions in rabbits with hind limb ischemia. On the other hand, adipose stromal cells (ASCs) have reparative effects in diverse tissues, mainly due to secretion of angiogenic factors like VEGF. Hence, we hipothezised that in this animal model ASCs modified to overexpress VEGF165 would exert greater anti-ischemic effect than non-modified ASCs. Rabbits with experimental left hind limb ischemia were injected with 10 million modified ASCs (ASC-VEGF), non-modified ASCs (ASC) or PBS (placebo) (n=10 per group). At 30 days, collaterals were counted (angiography) and muscle tissue samples were harvested for calculation of microvascular density and assessment of ischemic lesions. As compared with placebo, ACS-VEGF showed more collaterals (5.6±1.1 vs. 4.5±0.6 per cm²; p
