INVESTIGADORES
BERGUER Paula Mercedes
congresos y reuniones científicas
Título:
- Toll-Like Receptor 4 modulates the immune response against Brucella spp. Lumazine Synthase.
Autor/es:
BERGUER P, MUNDIÑANO J, PIAZZON I AND GOLDBAUM F
Lugar:
Rio de Janeiro, Brazil
Reunión:
Congreso; 13th International Congress of Immunology; 2007
Resumen:
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Brucella
spp. lumazine synthase (BLS) is a
highly immunogenic decameric protein, being an ideal scaffold for the
engineering of vaccines. We have shown that BLS activates dendritic cells (DCs)
in vitro and recruits DCs in vivo, being these effects dependent
on Toll-Like receptor 4 (TLR4). Here, we show that subcutaneous inoculation of BLS in BALB/c mice induced an increase not
only in the absolute number of DCs, but also of B, CD8+ and CD4+
lymphocytes in the draining lymph node. The percentages of activated B cells
and CD8+ cells (CD69+/CD19+ and CD69+/CD8+) increased 24h after BLS
inoculation. At 48h, the percentage and absolute number of CD44hi/CD8+ cells were also augmented in BALB/c mice. After
BLS inoculation, cultured lymph node cells were able to proliferate upon BLS
re-exposure, as assessed by [3H]thymidine incorporation. These events were dependent on
TLR4, as none of them were observed in mice lacking a functional TLR4 (C.C3Htlr4lps-d). The
level of IL-4 mRNA was significantly higher in the draining lymph node of
C.C3Htlr4lps-d mice than in BALB/c mice, as assessed by
semiquantitative RT-PCR. Although
total anti-BLS IgG levels were similar in both mice strains
intraperitoneally inoculated with BLS, IgG1/IgG2a ratio was higher in C.C3Htlr4lps-d.
These results show that BLS induces alterations in DCs, B cells and CD8+
cells, and is able to produce lymph node cell proliferation without adjuvant,
being these events dependent on TLR4. They also suggest that TLR4 signalling
favours a Th1 immune response.
