IIBBA   05544
INSTITUTO DE INVESTIGACIONES BIOQUIMICAS DE BUENOS AIRES
Unidad Ejecutora - UE
congresos y reuniones científicas
Título:
NDVB PROTEIN GENE IS INVOLVED IN XANTHOMONAS CAMPESTRIS (XC) GLUCAN BIOSYNTHESIS AND PATHOGENICITY
Autor/es:
RIGANO, L.A.; TORRES, P.S.; MALAMUD, F.; MARANO, M.R.; CASTAGNARO, A.P.; VOJNOV, A.A
Lugar:
Rosario, Santa Fe, Argentina
Reunión:
Congreso; XLII Reunion Anual, Sociedad Argentina de Investigaciones en Bioquimica y Biologia Molecular; 2006
Resumen:
Xc is a major bacterial pathogen of cruciferous plants worldwide.
The ability of Xc to incite disease in plants depends in part of the
production of extracellular factors such as degradative enzymes,
exopolysacharide, etc. An extracellular molecule also produced
by Xc, the cyclic glucans, have been shown to be important for a
number of plant-microbe interactions. We have identified a gene
involved in cyclic beta glucan biosynthesis of in Xc. The orf
YP245226 (identified as NdvB protein, an smaller version of the
Sinorhizobium NdvB gene) was disrupted through a cassete in a
site directed mutagenesis, and the insertion was confirmed by
PCR and Southern blot. The insertional inactivation 1,2 glucan,
assayedâof NdvB protein compromised the production of the
cyclic by size exclusion cromatography and TLC. The mutant
strain shows a significative impairment in the bacterial growth in
plant respect to the wild type strain.When the plant was
inoculated with the mutant it showed an exacerbated defense
response (PR1 transcript accumulation, callose deposition)
compared with the wild type inoculated plants. The production of
cyclic glucan and pathogenicity were fully restored by
complementation of the mutant with the Xc NdvB protein gene.
This results suggest an important role of the YP245226 orf in
xanthomonas glucan biosynthesis and pathogenicity.