In Vitro experiments of Superparamagentic Iron-Oxide Nanoparticles functionalized with PEGlated phospholipds

VASQUEZ MANSILLA, MARCELO; LIMA, ENIO; MOJICA PISCIOTTI M. L.; TROIANI, HORACIO E.; ZYSLER, ROBERTO D.; SILVA A. H; CRECZYNSKI-PASA T. B.; ZOLDAN V.; PASA A. A.; TORRES T. E.; CALATAYUD M. P.; SANZS B.; GOYA G. F.; ODDONE N.; BENECH J. C.

Buenos Aires

Workshop; X Latin American Workshop on Magnetism, Magnetic Materials and their Applications; 2013

CAC-CNEA/UBA

Superparamagnetic Iron-Oxide Nanoparticles (SPIONs) present a variety of applications in biomedicine. Thus, the production of SPIONs with controlled structural and magnetic properties are of great interest. At the same time, the functionalization of the nanoparticles is a key point, since it reflect in the hydrodynamic radius, the biocompatibility of the system and its superficial chemistry that determines the interaction of the nanoparticles with the biological medium. In the present work, we performed in vitro experiments of 18 nm SPIONS chemically synthesized on four distinct cell cultures. The nanoparticles were synthesized by chemical route at high-temperature, presenting a high-crystallinity and a narrow lognormal diameter distribution with = 18 nm and sigma = 0.10. The system present a high saturation magnetization and an effective anisotropy energy barrier that leads a blocking temperature slightly lower than room temperature for dc measurements. The SPIONs were functionalized with PE-PEG350 and PE-PEG2000. We have studied the citotoxicity of these functionalized SPIONs in four cell lineages: VERO, MDCK, NIH and neuroblasts. We determined by magnetic measurements the cell uptake dynamics for the four cultures and the localization of the nanoparticles in the cell were determined by FIB-SEM and co-focal microscopy. We have also performed AFM images of living cells and determined the changes of the elasticity of the membrane as consequence of the presence of nanoparticles. Finally, we found that the SPIONs-PE-PEG2000 were non-toxic for concentrations as high as 200 mu{SPIONs}mL and present a high cell up take rate in comparison to the SPIONs coated with PE-PEG350. Finally, we observed that the PE-PEG200 coated SPIONs are predominantly localized in the cytoplasm of the cell, while those coated with PE-PEG350 are attached to the cell membrane.