INVESTIGADORES
VENTURINO Andres
congresos y reuniones científicas
Título:
Pesticide effects on protein phosphorylation patterns in fish
Autor/es:
FERRARI A; VENTURINO A; PECHEN DE D'ANGELO A.M
Lugar:
Villa Carlos Paz
Reunión:
Congreso; XXXVIII Reunión Anual de la SAIB; 2002
Institución organizadora:
SAIB
Resumen:
Subacute and long-term effects of pesticide exposures
involve molecular effectors in the signaling transduction and genetic response
lo the stress. Protein kinase/phosphatase cascades play essential roles in the
toxicity of pesticides and organic pollutants. We present data on hepatic and
cerebral protein in vitro phosphorylation of juvenile rainbow trout
(Oncorhynchus mykiss) exposed to 0.005 mg/L of azinphosmethyl or 3 mg/L of
carbaryl. Nuclear and mitochondrial synaptosomal fractions were analyzed by
incubating them with 32P(gama)ATP and with ortovanadate (ATPase inhibitor) or
genistein (tyrosine-kinase inhibitor), followed by SDSPAGE and
autoradiography. A visible reduction of phosphorylation in an hepatic 64-Kda
protein band in the nuclear fraction was observed with both insecticides, as
well as an increase in 171-kDa protein band, wilh 01' without inhibitors.
Fishes exposed to azinphosmethyl present also an increase in phosphorylation of
a 22-kDa protein, which is more noticeable with ortovanadate. Carbaryl
exposure produces an attenuation of 22-kDa band phosphorylation in brain
synaptosomal fraction while it augments in nuclear fraction, suggesting a
probable protein relocalization. Coincidently with the effect observed in
liver, phosphorylation in 64-Kda band decreases while an additional
phosphorylaled protein (162-kDa) appears. On the other hand azinphosmethyl
produces also an increase in the phosphorylation of 22-kDa protein in brain
when ortovanadate was present. These preliminary results showing alterations
in the kinase- phosphatase pathways due to pesticide exposure imply changes in
signal transduction or' cellular dysfunctions, which might be related to
toxicological actions and/or responses to xenobiotic stress.