2D-DIGE protein expression analysis of melanoma cells: Assessing

FERNÁNDEZ ELMER A.; GIROTTI ROMINA; LLERA ANDREA S; PODHAJCER OSVALDO L; CANTET RODOLFO; BALZARINI MÓNICA

2007

Congreso; Sociedad argentina de Genetica; 2007

One of the main objectives when using 2D-DIGE for protein expression analysis is the detection of proteins potentially useful as biomarkers or involved in some path-ways. Since their appearance the usual techniques to discover differentially expressed proteins were the use of standard techniques such as fold-changes and t-test. Neither of them account for non-biological experimental effects and some ad-hoc techniques where developed and applied to achieve variance homogenization of the observed data.