BIOACTIVITY OF Zuccagnia punctata COMPOUNDS ON Fusarium verticillioides

JIMENEZ, C.M.; SAMPIETRO, D.A.; SGARIGLIA, M.A.; SOBERON, J.R.; VATTUONE, M.A.

SAN MIGUEL DE TUCUMAN

Congreso; XXIX ANNUAL SCIENTIFIC MEETING OF TUCUMAN BIOLOGY ASSOCIATION; 2012

TUCUMAN BIOLOGY ASSOCIATION

Fusarium verticillioides is an ethiological agent of the ear rot disease. It contaminates maize grains with fumonisins which are potentially toxic for humans and animals. We demonstrated that an ethereal fraction of Zuccagnia punctata (Fabaceae) tincture inhibits the growth of this pathogen. The aim of this work was to isolate and identify the responsible bioactive compounds. Aerial part tincture of Z. punctata was evaporated and partitioned with ethyl ether. The ether extract (EEt) was fractionated by chromatography in a Silica gel (230-400 Mesh) column (SCC). The collected fractions were grouped into 5 pools (G1, G2, G3, G4 and G5) according to their absorbance at 305 nm and TLC on Silica gel using UV at 365 and 254 nm, and NP-PEG. Bioactive components were detected by bioautography of TLC on a spore suspension of F. verticillioides. Bioactive bands were identifi ed using physical, chemical and spectroscopy detection methods. Results were compared with others obtained with pure standards. Antifungal constituents of EEt were detected in G2, G3, G4 and G5. Analyses performed suggest that these compounds are fl avonoids. Two of them were isolated and identifi ed as 2?,4?-dihydroxychalcone and 2?,4?-dihydroxy-3?-metoxichalcone. The purifi cation and structural elucidation of unidentifi ed bioactive molecules is in progress.