INVESTIGADORES
CIANCIA Marina
congresos y reuniones científicas
Título:
System of polysaccharides of Codium decorticatum from the Patagonic coast
Autor/es:
MARINA CIANCIA; MARÍA PAULA RAFFO; PAULA VIRGINIA FERNÁNDEZ
Lugar:
Nusa Dua, Bali
Reunión:
Simposio; 21th International Seaweed Symposium; 2013
Resumen:
System
of polysaccharides of Codium decorticatum
from the Patagonian coast
Fernández PV1,
Raffo MP2 , Ciancia M*,1,3
1Cátedra de Química de Biomoléculas, Departamento de
Biología Aplicada y Alimentos, Facultad de Agronomía, Universidad de Buenos
Aires, Argentina ciancia@agro.uba.ar
2CENPAT-CONICET, Puerto Madryn, Chubut, Argentina
3CIHIDECAR-CONICET, Departamento de Química Orgánica,
Facultad de Ciencias Exactas y Naturales, Universidad de Buenos Aires,
Argentina.
Codium seaweeds, biosynthesize sulfated galactans
constituted by 3-linked b-D-galactopyranose residues
partially sulfated on C-4 and/or C-6, with ramifications on C-6 and important
amounts of pyruvate [1,2]. Besides, a
highly sulfated pyranosic arabinan [2] and a 4-linked mannan, partially sulfated
on C-2 [3], were also found. Codium
decorticatum was collected in Puerto Madryn (Chubut, Argentina).
The polysaccharides sulfated were obtained by water extraction at room
temperature (D1 and D2) and then, at 90ºC (C1 and C2). The residue from the water
extraction procedure was constituted by mannans (96 %). Analysis of the
extracts showed that galactose and arabinose are the most important sugar
components of D1 and D2, while C2 is constituted mainly by mannose and smaller
amounts of glucose. All the extracts contained sulfate (23-10 %, as SO3Na).
Attempts were made to fractionate D1 by precipitation with increasing KCl concentration,
but most of the sample remained soluble in 2.0 M KCl solution and all
the fractions contained similar amounts of the major monosaccharides. However,
fractionation by ion exchange chromatography gave a fraction with 88 % of
galactose (Fg) and another which contained 74 % of arabinose (Fa). Structural
analysis of Fg showed the structural features characteristic of galactans of
other species of Codium, with
predominance of 4-sulfated units. Fa showed an arabinan structure, mainly
sulfated on C-2. Fa will be tested as anticoagulant, taking into account that
previous results showed the importance of sulfation on C-2 on the direct
inhibition of thrombin [2].
[1] Bilan MI, Vinogradova EV, Shashkov
AS, Usov AI, Carbohydr. Res. 342 (2007) 586?596. [2] Fernández PV, Quintana I Cerezo AS, Caramelo JJ, Pol-Fachin L, Verli H,
Estevez JM, Ciancia M, J. Biol. Chem. In press. http://www.jbc.org/cgi/doi/10.1074/
jbc.M112.386441. [3] Fernández PV, Estevez JM, Cerezo AS, Ciancia M,
Carbohydr. Pol., 87 (2012) 916-919.
Category of presentation: Poster