Comparison of DNA fragmentation dynamics in frozen sperm of Equus asinus between uncentrifuged and SLC selected samples

ISABEL ORTIZ; JESUS DORADO; JANE MORREL; JAVIER GOSALVES; FRANCISCO CRESPO; LUISA RAMIREZ; DANIEL ACHA VALLS; MAYTE URBANO; MARIA JOSE GALVES; SEBASTIÁN DEMYDA PEYRÁS; MANUEL HIDALGO

Bolonia

Congreso; 17th annual European Society for Domestic Animals Reproduction Conference; 2013

European Society of Domestic Animal Reproduction

Single layer centrifugation (SLC) has been used to decrease the percentage of sperm DNA fragmentation of equine frozen semen. However, no studies on the effect of SLC on DNA fragmentation have been performed in donkeys. The aim of this study was to compare the rate of increase of DNA fragmentation expressed as the slope of the dynamic regression line of frozen donkey semen between unselected and SLC samples. Sperm DNA fragmentation (sDF) was assessed using an adaptation of the sperm chromatin dispersion test developed for stallions in five donkeys. SLC was performed after thawing using Androcoll-E in one aliquot of each ejaculate. Sperm samples were thawed and incubated at 37°C and sDF was assessed at 0, 3, 6 and 24 h of incubation. Comparison of the medium values of the slope of unselected and SLC groups were statistically compared by ANOVA. The medium slope of the regression line of sDF was significantly lower in the SLC group (p < 0.05) in comparison to unselected samples (0.64 0.34 vs. 1.25 0.36). A lower slope indicates higher DNA stability. In conclusion, SLC using Androcoll-E decreases the slope and velocity of DNA fragmentation of frozen-thawed donkey semen.