Influence of sperm capacitation media used on the percentages of numerical chromosomal abnormalities on early in vitro produced bovine embryos

SEBASTIÁN DEMYDA PEYRÁS; JESUS DORADO; DANIEL ACHA VALLS; ISABEL ORTIZ; MARIA JOSE GALVES; LUISA RAMIREZ; MAYTE URBANO; MANUEL HIDALGO; MIGUEL MORENO MILLÁN

Bolonia

Congreso; 17th annual European Society for Domestic Animals Reproduction Conference; 2013

European Society of Domestic Animal Reproduction

Chromosomal aberrations (CA) are widely related to retarded development, embryo failure and fetal losses in the domestic animals. It is also widely demonstrated that the percentage of CA is higher when the embryos are produced in vitro. Recent studies regarding the causes of such abnormalities are scarce. Thus, this cytogenetic study was conducted to quantify the incidence and type of chromosomal abnormalities on in vitro-produced (IVP) bovine embryos produced using spermatozoa capacitated in three different media (Brackett Oliphant (BO), TCM-199 and Sp-TALP). Oocytes were matured using our standard laboratory techniques (TCM-199 for 18?20 h; 38.5°; 5% CO2). Thereafter, oocytes were divide in three groups and fertilized using sperm previously capacitated for 1 h at 38.5° in three different capacitation media. Presumptive zygotes were cultured for 72 h, collected and disaggregated into their blastomeres on individual slides under a stereomicroscope at 409 magnification. Thereafter, each embryo were fixed individually and stained with Giemsa. Numerical chromosomal abnormalities were determined by direct observation at 12509 magnification. During our study, a total of 174 embryos were suitable for karyotyping. The percentages of total abnormalities and the rates of haploidy, diploidy, aneuploidy and polyploidy were determined individually. The percentages of normal diploid embryos were 90.1% for BO; 90.9% for TCM-199 and 87.9% for Sp-TALP. No significant differences were observed when each kind of chromosomal abnormality was analyzed separately. According to this results, no differences were observed among treatments (p > 0.05). Hence, variations in the composition of the capacitation media do not seem to produce any effect in the chromosomal abnormality rates in the derived in vitro produced embryos.