Effect of egg yolk on the kinematics and acrosome membrane integrity of cooled-rewarmed canine spermatozoa.

JESUS DORADO; MARIA JOSE GALVES; MARIA RITA MURABITO; SEBASTIÁN DEMYDA PEYRÁS; LEONARDO DE LUCA; MIGUEL MORENO MILLÁN; MANUEL HIDALGO

Cordoba, Argentina

Congreso; 36th International Embryo Transfer Society Conference; 2010

International Embryo Transfer Society

Tris-egg yolk-based diluents provide adequate cryoprotection for the sperm of most species. This study was conducted to compare the ability of Tris-glucose extender containing 2 different concentrations of egg yolk to maintain sperm motility and acrosome integrity of canine spermatozoa during 72 h of preservation. For this purpose, a total of 20 ejaculates from 4 clinically healthy dogs (2 Spanish Greyhound, 1 German Pointer, and 1 Crossbreed) were collected by digital manipulation. The sperm-rich fraction of each ejaculate was divided into 2 aliquots. Then, they were diluted in Tris-based extender and centrifuged at 700g for 8 min. Sperm pellets were resuspended in eitherTris buffer added to 20% (EY20) or 10% centrifuged egg yolk (EY10) and cooled to 5◦C over 72 h. The effects of these extenders on motility and acrosome integrity were assessed objectively using a computer-aided semen analyzer (Sperm Class Analyzer, Microptic SL, Spain) and Spermac® staining, respectively. Each cooled-rewarmed semen sample was evaluated after 24, 48, and 72 h of preservation. Sperm motion parameters shown by computer-assisted semen analysis (CASA) are progressively motile (PMS) and motile spermatozoa (MS), curvilinear velocity (CLV), average path velocity (APV), progressive speed (SLV), and lateral head displacement (LHD). Datawere statistically analysed byANOVA. Dependent variables expressed as percentageswere arsine-transformed before analysis. Differences between mean values were evaluated by the Duncan method. Data were presented as mean±SEM. Differences were considered significant when P