Nuclear and cytoplasm abnormalities and metalloproteases expression in the mouse embryo-trophoblast-decidual tissues at time of gastrulation and after periconceptional alcohol consumption

PEREZ TITO L; COLL ,T.A.; CEBRAL E

Iguazu Falls-Parana

Congreso; V SLIMP- Latin American Symposium on Maternal-Fetal Interactions & Placenta and IV Latin American Symposium on Reproductive Immunology; 2013

SLIMP

Nuclear and cytoplasmic abnormalitiesand metalloproteases expression in embryo-trophoblast-decidual tissues on gastrulation after periconceptional alcohol consumptionin mouse.  Pérez Tito, L.G.; Coll, T.A.; Cebral, E.  LARFIMAE, IFIBYNE-CONICET, Dpto. BBE, Facultad de Ciencias Exactas y Naturales, Universidad de Buenos Aires.  Murine perigestational alcohol ingestion until day 10 of gestation (GD) increases resorption at DG7-8. We postulate a link with MMP-2 and -9 expression changes and cellular alterations on GD8 implantation sites (IS). Ethanol/water-10% was administered to CF-1 females 17 days before and up to GD8 (TF) (Control Females, water). Differentiation stage, histopathology, apoptotic index, collagen-IV and MMP-2 and -9 immunoexpression were evaluated in decidua (De), ectoplacental cone (EC), trophoblast-decidual interface (TDInt), embryo and extraembryonic tissues. TF showed 24h of differentiation delay. Non-delayed-TF-De had decreased collagen-IV, altered MMP-2 and -9 expression and increased TUNEL+cells. TF-EC-Proliferative area was lower and MMP-9 increased. TF-TDIn had histological abnormalities and increased erythrocyte infiltration, apoptosis and MMP-9 immunoexpression. TF-embryos (66%) had abnormal neuroectoderm with high MMP-2 and -9. Periconceptional alcohol exposure produces histological defects, high cell death and collagen-IV, MMP-2 and -9 expression changes at GD8, suggesting that these factors participate on early embryonic loss induction.