Experiments with functionalized SPIONs for applications in hyperthermia: analysis of the response to an alternating magnetic

M. L. MOJICA PISICOTTI; E. LIMA JR.; R. D. ZYSLER; M. VASQUEZ MANSILLA; H. E. TROIANI; G. F. GOYA

Buenoa Aires

Workshop; Latin America Workshop on Magnetism, Magnetic Materials and their Applications; 2013

In nanomedicine, the hyperthermia is one of the most promising procedures involving diagnosis and treatment of cancer. This technique consists of inducing cell death by increasing the local temperature in a tumor tissue. This heating is mediated by superparamagnetic iron oxide nanoparticles (SPIONs) that are absorbed by cancer cells and thus they interact with an AC magnetic eld in such way that the SPIONs receive energy from the eld and then convert it into thermal energy. This aggressive increase of the temperature cause their death.[1] Then it is desirable to design and produce SPIONs with controlled diameter and small size distribution. Moreover, the eectiveness of hyperthermia is focused on using SPIONs with a high biocompatibility and optimized magnetic properties. In the present work we have synthetized Fe3O4 SPIONs through the method of high temperature decomposition of iron acetylacetonate (Fe(acac)3) which oers an optimal control over size and dispersion. We obtained well-crystalline SPIONs of dierent sizes and we studied their morphological and magnetic properties. The nanoparticles exhibited high saturation magnetization and superparamagnetic behavior at room temperature.[2;3] Once hydrophobic SPIONs were obtained, they were suspended in aqueous media by the method of ligand exchange.[4] Through an alternative method, the as-made nanoparticles were coated with polyethlylene glycol- (PEG-) and DEXTRAN- 11-aminoundecanoic acid tetramethylammonium salt. Specic absorption rate (SAR) measurements were performed on a commercial device (200 Oe with dierent frequencies). These measurements showed high SAR values, which suggest that SPIONs treated with the method described above are appropriate for hyperthermia experiments. Also, it was found an expected dependence with concentration in all the cases, as well as with the media where they were suspended. [1] Hergt R. et al. J.Phys: Condens. Matter 18 (38), S2919-S2934 (2006) [2] Vargas J.M. and Zysler R.D. Nanotechnology 16, 1474-1476 (2005) [3] Sun S. and Zeng H. J. Am. Chem. Soc. 124(28), 8204-8205 (2002) [4] Shivang R.D. and Gao X. Wiley Interdisciplinary Reviews: Nanomedicine and nanobiotechnology 1(6), 583-609 (2009)