IIBBA   05544
INSTITUTO DE INVESTIGACIONES BIOQUIMICAS DE BUENOS AIRES
Unidad Ejecutora - UE
congresos y reuniones científicas
Título:
Conditional suppression of action potential firing of pacemaker neurons uncouples the molecular clock from circadian outputs in adult Drosophila
Autor/es:
DEPETRIS-CHAUVIN A; BERNI J; ARANOVICH EJ; MURARO NI; BECKWITH EJ; CERIANI MF
Lugar:
Destin, FLORIDA
Reunión:
Congreso; 13th Biennial Meeting, Society for Research on Biological Rhythms; 2012
Institución organizadora:
Society for Research on Biological Rhythms
Resumen:
In
Drosophila, circadian rhythms are regulated
through transcriptional feedback loops of clock genes running within specific pacemaker cells. In
particular, molecular oscillations in the small ventral Lateral Neurons (sLNvs)
command rhythmic behavior under free-running conditions releasing the
neuropeptide PIGMENT DISPERSING FACTOR (PDF) in a circadian fashion. It has
also been established that electrical activity in the sLNvs is required for
behavioral rhythmicity. However, the consequences of electrical silencing of
pacemaker neurons over both core molecule oscillations and clock outputs remain
controversial. Genetic manipulations throughout development have been shown to
produce compensatory changes and therefore are likely to cause additional undesired
effects. We developed a new genetic tool for spatiotemporal control of gene
expression to obtain adult-restricted electrical silencing of the PDF circuit,
which led to reversible behavioral arrhythmicity. Remarkably, PER oscillations
during the silenced phase remained unaltered, indicating that arrhythmicity is
a direct consequence of the silenced activity. As expected, circadian axonal
remodeling and PDF accumulation were severely affected during the silenced
phase. These data suggests that sLNv electrical activity is not a core clock component
but a circadian output involved in the coordination of rhythmic locomotor behavior.