Glyphosate herbicide increase hepatic pigmentation in Leptodactylus latinasus: protective effect of melanin

PÉREZ IGLESIAS J.M.; FRANCO-BELUSSI, L.; DE OLIVERA C.; NATALE G.S.

Pernambuco

Congreso; XII Congresso Brasileiro de Ecotoxicologia, Porto do Galinhas, Pernambuco, Brasil; 2012

SETAC LA

Melanomacrophages are pigmented cells with macrophagic activity and they are found in hematopoietic organs. These cell types are found in the liver of amphibians and have different types of granules in the cytoplasm, besides melanin, that results from cellular catabolism. Hemosiderin is one of those substances, derived from erythrophagocytosis. Another substance is lipofuscin, which seems to be derived from damaged cellular components, through the peroxidation of their unsaturated lipids. The functional role of these visceral pigmented cells is poorly known. However, melanomacrophages has been hypothesized to act in pollens detoxification. We analyzed the response of melanomacrophages in the liver of the frog Leptodactylus latinasus administrated to 20 adult males with different concentrations of Glyphosate ? GLY (active compound), for 48h. The concentrations of GLY were: 100mg/L(GLY100), 1000 mg/L (GLY1000), 10000 mg/L(GLY10000) and a control group (GLY0) administrada con una inyeccion (una dosis unica al tiempo 0) via intraperitoneal We conducted morphological and histochemical analyses. For histological analyses we utilized histological section stained with HE. And for histochemical analyses we use Schmorl?s solution and acid solution of ferrocianide for lipofuscin and hemosiderin detection respectively. Melanocrophages in the liver are rounded in shape, and posses melanin, hemosiderin and lipofuscin inside. The pigmentation in the liver increased in the GLY100 (P