COMBINED ANTIFUNGAL ACTIVITY OF TRIPODANTOSIDE AND AMPHOTERICIN B AGAINST Candida SPECIES YEASTS

SOBERÓN, J.R.; SGARIGLIA, M.A.; SAMPIETRO, D. A.; VATTUONE, M.A.

Tafi del Valle

Congreso; XXVIII Annual scientific meeting of the Tucuman biology association; 2011

Tucuman biology association

Fungal infections caused by yeast pathogens are common in immunocompromised patients. Candida spp. represents the major group of yeasts recovered from these patients. The limited number of available antifungal drugs (azoles, polyenes and pyrimidine analogues),their toxicities and resistance make it necessary to find new antifungal drugs. The main constituent of Tripodanthus acutifolius (Ruiz & Pavon) van Thieghem leaves is tripodantoside (TP), a phenylbutanoid with no data about its antifungal effects. The antifungal activities of TP and amphoter icin B (AMB), both individually and combined, were assayed agai nst C albicans (ATCC I023 1) an d C tropicalis (ATCC750) using the broth microdilution method. TP showed inhibitory effects against both strains at 20 ug/ml. The association of TP+AMB resulted in additive effects against both strains when combined assays were performed. The combination of TP+AMB could diminish the AMB therapeutic doses and the toxicity associated with the use of AMB. The antifungal activity of TP supports the need for further studies of its action modes.