Bioactivity acute toxicity test using as experimental model bacteria

MASCOTTI ML; BISOGNO FR; KURINA SANZ MB; GIANNINI FA

Potrero de los Funes San Luis Argentina

Congreso; XXIV REUNIÓN CIENTÍFICA ANUAL DE LA SOCIEDAD DE BIOLOGÍA DE CUYO; 2006

Sociedad de Biologíade Cuyo

139   Bioactivity acute toxicity test using as experimental model bacteria. Mascotti ML, Bisogno F, Kurina M,, Giannini F. Fac. De Qca, Bqca y Fcia. UNSL. e-mail: fagian@unsl.edu.ar   The potential bioactivity of a compound can be associated with it’s toxicity, taking into account the noxious effect that drugs presents toward living organisms. This evaluation can be done using multicellular organisms as crustaceans, rotifers, fishes, amphibians and even for more advanced studies mammalian; but those assays are very expensive, requires important quantities of drugs and even are very conflictive because of animals law’s protection. We present in this work the development of a bioassay requiring bacteria as experimental model, specifically Lactobacillus acidophilus and Bacillus subtilis. Commercial lyophilized bacteria are suitable for this assay. As a primal stage of this work we standardized conditions that direct the assay as amount of initial inocula, optimal incubation time, culture media and positive controls. The obtained results allow us to present it as a valid, fast, economic, reproducible tool, easy to handle assay, requiring low amount of tested compounds,  in order to measure potential drug bioactivity and toxicity.