IIBBA   05544
INSTITUTO DE INVESTIGACIONES BIOQUIMICAS DE BUENOS AIRES
Unidad Ejecutora - UE
congresos y reuniones científicas
Título:
THE TWO-COMPONENT SYSTEMS PrrBA AND NtrYX REGULATE THE ADAPTATION OF Brucella TO LOW OXYGEN TENSION
Autor/es:
CARRICA, MARIELA DEL CARMEN; FERNÁNDEZ, IGNACIO; PARIS, GASTON; GOLDBAUM, FERNANDO
Lugar:
Mendoza
Reunión:
Congreso; XLVII Reunión Anual Sociedad Argentina de Investigación en Bioquímica y Biología Molecular; 2012
Institución organizadora:
Sociedad Argentina de Investigación en Bioquímica y Biología Molecular
Resumen:
Brucella abortus is the causative agent of the zoonotic disease brucellosis, which is endemic in many parts of the world. The success of Brucella as pathogen resides in its ability to adapt to the harsh
environmental conditions found in mammalians host. One of its
main adaptations is the induction of the expression of different
genes involved in respiration at low oxygen tension. We describe a
regulatory network involved in this adaptation. We show that in Brucella abortus the two-component system PrrBA regulates the
expression of the two-component system NtrYX, which is
involved in redox sensing, and the transcriptional regulators FnrR
and NnrA, which are involved in oxygen and nitric oxide sensing,
respectively. We also show that PrrBA and NtrYX coordinately
regulate the expression of denitrification and high-affinity
cytochrome oxidase genes. Strikingly, a double mutant strain in prrB
and ntrY genes is severely impaired in growth and virulence,
while the and single mutant strains are not. The
regulatory network proposed could contribute to shed light into the
mechanisms used by for a successful adaptation to its
replicative niche inside mammalians cells.