Effect of single-layer centrifugation with equipure? on motility kinematics of frozen?thawed donkey sperm

ISABEL ORTIZ; JESUS DORADO; DANIEL ACHA VALLS; LUISA RAMIREZ; MAYTE URBANO; MARIA JOSE GALVES; LUCRECIA ALCARAZ; JOSE MANUEL PORTERO; FABIOLA QUESADA; CARLOS GONZALES; SEBASTIÁN DEMYDA PEYRÁS; MANUEL HIDALGO

Hannover

Congreso; 39th International Embryo Transfer Society Anual Meeting; 2013

IETS (International Embryo Transfer Society)

Single-layer centrifugation (SLC) with EquipureTM Bottom Layer has been used to enhance the quality of stallion semen samples; however, no studies have been performed on donkeys. The aim of this study was to determine if SLC with EquipureTM Bottom Layer improves kinematic parameters on frozen?thawed donkey sperm. Semen was collected from 4 Andalusian donkeys by artificial vagina. Three ejaculates from each donkey were centrifuged with EquiproTM, supernatant was removed, and pellet was re-extended in the freezing medium GentTM to a final concentration of 200 × 106 spermatozoa per milliliter. Sperm were slowly cooled to 5°C for 2 h, loaded in 0.5-mL plastic straws, and frozen in liquid-nitrogen vapors. After at least one week of storage, straws were thawed in a water bath at 37°C for 30 s. After thawing, semen samples were divided in 2 aliquots: aliquot 1 was used as such (control) and aliquot 2 was processed by SLC using EquipureTM Bottom Layer. Computer-assisted sperm analysis was performed, and sperm kinematics total motility (%), progressive motility (%), curvilinear velocity (VCL; µm s?1), velocity straight line (VSL; µm s?1), velocity average path (VAP; µm s?1), linearity (LIN; %), straightness (STR; %), wobble (WOB; %), lateral head displacement (ALH; µm), and beat cross frequency (BCF; Hz) were statistically compared using GLM model between frozen?thawed semen samples processed or not with EquipureTM. Results were expressed as mean ± standard error. Significant differences (P