Filling dynamics of the Brindley?s glands of the haematophagous bug Triatoma infestans (Hemiptera, Reduviidae) and associated behavioural responses

PALOTTINI F.; GONZÁLEZ, A.; MANRIQUE, G.

Vilnius

Congreso; International Society of Chemical Ecology 28th annual meeting; 2012

ISCE

<!-- /* Style Definitions */ p.MsoNormal, li.MsoNormal, div.MsoNormal {mso-style-parent:""; margin:0cm; margin-bottom:.0001pt; mso-pagination:widow-orphan; font-size:12.0pt; font-family:"Times New Roman"; mso-fareast-font-family:"Times New Roman"; mso-ansi-language:EN-GB; mso-fareast-language:EN-US;} h2 {mso-style-link:" Car Car"; mso-style-next:Normal; margin:0cm; margin-bottom:.0001pt; mso-pagination:widow-orphan; page-break-after:avoid; mso-outline-level:2; font-size:12.0pt; font-family:"Times New Roman"; mso-ansi-language:X-NONE; mso-fareast-language:X-NONE; font-weight:normal; font-style:italic;} span.CarCar {mso-style-name:" Car Car"; mso-style-locked:yes; mso-style-parent:""; mso-style-link:"Título 2"; mso-ansi-font-size:12.0pt; mso-bidi-font-size:12.0pt; mso-ansi-language:X-NONE; mso-fareast-language:X-NONE; mso-bidi-language:AR-SA; font-style:italic;} @page Section1 {size:595.3pt 841.9pt; margin:70.9pt 70.9pt 70.9pt 70.9pt; mso-header-margin:35.45pt; mso-footer-margin:35.45pt; mso-paper-source:0;} div.Section1 {page:Section1;} --> Filling dynamics of the Brindley?s glands of the haematophagous bug Triatoma infestans (Hemiptera, Reduviidae) and associated behavioural responses   Palottini F1, González A2, Manrique G1   1 Laboratorio de Fisiología de Insectos, Departamento de Biodiversidad y Biología Experimental, Facultad de Ciencias Exactas y Naturales, Buenos Aires, Argentina 2 Laboratorio de Ecología Química, Facultad de Química, Universidad de la República, Montevideo, Uruguay   florpal@bg.fcen.uba.ar   When adults of Triatoma infestans are mechanically disturbed, Brindley´s glands emit its volatiles, which are postulated as alarm pheromone and evoked escape behaviour of larvae. This work studied the filling dynamics of the Brindley´s glands and determined whether the capacity to release the secretion is associated with feeding. In addition, we analyzed the relationship between this dynamics and behavioural responses of larvae. Adults were disturbed weekly, fed or not, their glands were dissected and then its contents were analyzed according to the fasting state using a gas chromatograph. Using a double choice olfactometer in behavioural tests, the orientation of larvae submitted to volatiles released by disturbed adults with varying degrees of fasting was analyzed. Not fed disturbed adults presented empty glands (1.7 g), while fed adults recharged them (11.3 mg). When larvae were exposed to odors from three time?s disturbed adults that were unfed, they did not show a direction opposite to the stimulus, whereas against odors from three time?s disturbed adults, posteriorly fed, they were oriented in a direction opposite to the stimulus. Taking as an indicator the isobutyric acid content, the filling dynamics of the glands depended on feeding. The fasting degree of adults would limit the recharge capacity of Brindley´s glands for an eventual release of the secretion to a new disturbance event that would be reflected in the escape behaviour of larvae.   Acknowledgments The research was supported by the ANPCyT (PICT01191), CONICET and Universidad de Buenos Aires.