In vitro endothelial cells? response to bioactive glass ionic dissolution products

HARO DURAND L, GONGORA A, GOMEZ M, PORTO LÓPEZ JM, BOCCACCINI AR, ZAGO MP, BALDI A, GORUSTOVICH A.

Mar del Plata

Workshop; Second Workshop on Artificial Organs, Biomaterials and Tissue Engineering; 2011

Sociedad Latinoamericana de Biomateriales, Ingeniería de Tejidos y Órganos Artificiales

Publicado en Biocell 2012;36(1):A4      The aim of the present study was to evaluate the direct response of endothelial cells to the ionic dissolution products (IDPs) of bioactive glasses (BGs). Human umbilical vein endothelial cells (HUVECs) seeded on 96-well tissue culture plates were treated with conditioned media (CM) containing the IDPs of BGs that were prepared by soaking 1% w/v 45S5 BG particles (< 5 µm) of composition (in wt %): 45% SiO2, 24,5% Na2O, 24,5% CaO, 6% P2O5 or 45S5.2B BG containing B2O3 (2% wt) in complete endothelial basal medium M199 in a orbital shaker at 37°C for 24 h. The elementary content of Ca, Si, B, P and Na in the medium were determined by ICP. Cell proliferation was quantitatively assessed using the [3H]thymidine incorporation assay. The migratory capacity of cells was evaluated by the wound healing assay. Cytokine expression was measured by an ELISA assay. Compared to controls, cell proliferation, migration and IL-6 release of HUVECs were statistically significantly increased by CM with BG 45S5.2B. The robust proangiogenic activity of soluble 45S5.2B BG degradation products appears to have promise for future application in tissue engineering and regenerative medicine.