Efficiency of encapsulation of beneficial lactic acid bacteria with the dripping method, and survival at different storage temperatures

MARIA ELENA FATIMA NADER; EZZINE ABDELAZIZ; MARIA LUJAN JIMENEZ PRANTEDA; MERCEDES MONTEOLIVA SANCHEZ; ALBERTO RAMOS CORMENZANA

Tucuman

Simposio; III Simposio Internacional de Bacterias Lácticas.; 2009

CERELA_CONICET

EFFICIENCY OF ENCAPSULATION OF BENEFICIAL LACTIC ACID BACTERIA WITH THE DRIPPING METHOD, AND SURVIVAL AT DIFFERENT STORAGE TEMPERATURES Maria Elena Fátima Nader-Macias1, Ezzine Abdelaziz2, Maria Lujan Jimenez Pranteda2, Mercedes Monteoliva Sanchez2 and Alberto Ramos Cormenzana2 1CERELA-CONICET, Chacabuco 145, T4000ILC, San Miguel de Tucumán. 2 Departamento de Microbiología. Facultad de Farmacia. Universidad de Granada. España. Email: fnader@cerela.org.ar Encapsulation in a method proposed to improve the survival of probiotics during their storage, both in foods and in pharmaceutical or veterinary applications, and also to increase their performance during their passage to the tract where they will be applied. In previous works, the isolation, identification and evaluation of the beneficial characteristics of microorganisms from different ecological niches, both human and animals were performed. The aim of this work was to evaluate if some of the beneficial microorganisms can be encapsulated in alginate comparatively with gellan and xanthan gums, when using the dropping method applied mainly for liquid or viscous applications. Also, to evaluate the survival of the microorganisms during the storage at different temperatures Six different probiotic microorganisms (10-11log CFU previously cultured in MRS or Laptg broths) concentrated in distilled water was added to 15 mL solution of 1% Xanthan gum + 0.75% Gellan gum or 2% sodium alginate. This mixture was extruded to form the beads through a syringe pump equipped adequately, and received in CaCl2. The flow rate was 120 ml/h. Viable cells number was determined by counting plates from beads, previously released with 0.05M phosphate buffer, pH=7 by using a stomacher. The beads were divided in aliquots, and stored in individual microtubes at -20, 4 and 25ºC The results show that the encapsulation efficiency obtained was between 60 and 100%, and different if alginate (A) or xanthan-gellan (X-G) gum was used.   Lactobacillus gasseri CRL 1307, CRL 1252 and L. reuterii CRL 1324, isolated from human vaginal tract, showed viable cells up to 15 days at 25ºC, while at 4ºC and at -20ºC, the viability was maintained for 50, 220 and 236 days, respectively, both in A or XG beads. The beads with L. johnsonii CRL 1693, isolated from calves faeces, showed viability only up to day 18 at all the temperatures evaluated, and in both bead types. Weisella CRL 1733 and E. mundtii CRL 1657, from bovine mammary gland, showed higher viability in XG than in A bead, both at 4 or -20ºC up to 208 days. The results obtained show that the microencapsulation of microorganisms is a method that can be applied for the long-term storage of microorganisms for pharmaceutical applications at low temperatures (-20 and 4ºC for XG beads, or only 4ºC for A beads), depending on each particular strains