Microsatellite analysis of the ING tumor suppressor genes and relationship with clinicopathological features in ameloblastoma

SILVIA S. BORKOSKY; MEHMET GUNDUZ; NAOKI KATASE; RYO TAMAMURA; HITOSHI NAGATSUKA

Okayama

Encuentro; 29th Annual Meeting of Okayama Dental Society; 2008

Okayama University, Graduate School of Medicine, Dentistry and Pharmaceutical Sciences

Purpose: To analyze by microsatellite analysis the loss of heterozygosity (LOH) status of the ING family of tumor suppressor genes (TSGs) in a group of ameloblastomas, and, to detect the correlation between the ING LOH status and clinicopathological characteristics. Methods: We performed LOH analysis, by designing the microsatellite markers ING1MS, ING2MS, ING3MS, ING4MS, ING5MS. These markers are located in close proximity to each ING gene family member. In addition the marker D2S140 (ING5 locus), available through the Internet genome database, was also used. Paired normal and tumor samples were obtained from 38 cases of ameloblastoma. After amplification of each primer by polymerase chain reaction (PCR), the samples were electrophoresed in 8% polyacrylamide gel, followed by visualization of DNA bands by silver staining. The correlation between the ING LOH status and clinicopathological characteristics was also evaluated, by using statistical methods. Results: High frequency of LOH was found for each ING gene family member (37.9-72.2%). Statistical significant association was obtained between solid tumor type and LOH of D2S140 (p= 0.02). LOH of ING3MS was also high in solid type tumors, showing a near significant association. In addition, a notable tendency toward higher LOH for most markers was observed in recurrent cases. Conclusions: LOH of ING family genes appears as a common genetic alteration in ameloblastoma. Moreover, ING LOH ratio in relation with tumor recurrence and aggressiveness suggests the potential prognostic significance and supports the tumor suppressor character of the ING gene family members in ameloblastoma.