Bone radiomorphometry obtained after treatment with bioactive glass and statins

ROSA AVELLANEDA; IRENE ALINCASTRO; SILVIA S. BORKOSKY; ERIKA TERRITORIALE; SILVIA KOZUSZKO; LILIANA R. MISSANA

San Miguel de Tucumán

Congreso; XXXIV Reunión Científica Anual de la Sociedad Argentina de Investigación Odontológica - División Argentina de la International Association for Dental Research (IADR); 2001

Sociedad Argentina de Investigación Odontológica

Radiological methods are very useful for skeletal studies. This technique is appropriate to recognize bone formation and resorption in experimental defects.  Radiomorphometry has been developed to measure radiopacity and radiolucity in order to understand the biological bone reparation process. In SAIO 2000 we demonstrated that Critical Size Defect (CSD) for rats tibia is 6x3x3 mm at 1 week after surgery. The aim of this work is to evaluate quantitatively radiopacity and radiolucity, obtained in CSD treated by bioactive glass (BG) with and without simvastatin (S). Fifty five radiographs were used from sprague dawley male rats (250 g) with CSD made under anesthesia at cortical tibial bone. The animals were killed after 1, 2, 3 and 6 weeks of treatment. The radiographs were divided in three groups: Group A control: non BG, neither S; Group B: filled with BG; Group C: filled with BG and injected by S. The simvastatin was intraperitoneal injected through five days (10mg/kg/day). The radiographs were projected onto screen and analysed using a lattice (256 points). The parameters selected were radiopacity and radiolucity areas. The results showed at 1 week, Group A: radiolucity 100% and radiopacity 0%. Group B: radiolucity 36% and radiopacity 63%. Group C: radiolucity 23% and radiopacity 76%. At six weeks radiopacity was 100% in all group studies. From these results we conclude that the CSD treated with bioactive glass and simvastatin demonstrate a high radiopacity in contrast with other groups.