Examination of loss of heterozygosity in 1p36 chromosomal region in oral squamous cell carcinoma

MATHIEU LEFEUVRE; MEHMET GUNDUZ; ESRA GUNDUZ; MAHMOUD AL SHEIKH ALI; NAOKI KATASE; SILVIA S. BORKOSKY; RYO TAMAMURA; HITOSHI NAGATSUKA

Tokio

Congreso; The 50th Annual Meeting of Japanese Association for Oral Biology; 2008

Japanese Association for Oral Biology

Despite surgical and radio-chemotherapeutic improvements for treatment of oral cancer, 50% of the patients still succumb to the disease. Unfortunately, few are known about genetic mechanisms of oral carcinogenesis. In this study, we investigated loss of heterozygosity (LOH) in 1p36 chromosome region, which has been previously reported to harbor various tumor suppressor genes (TSGs), in 27 patients with oral cancer using 9 polymorphic microsatellite markers. LOH was found at least in one locus in 85% of the cases. The markers D1S243 (25%), D1S468 (22%), D1S450 (25%), D1S228 (38%), D1S199 (28%) and D1S1676 (23%) showed higher LOH frequencies. Three preferentially deleted regions were identified in this study, including region 1 (D1S468-D1S243), region 2 (D1S450-D1S228) and region 3 (D1S199-D1S1676). Multiple candidate TSGs, such as RIZ1, p73, UBE4B, Rap1GAP, EPHB2 and RUNX3, are located in these three areas. Based on these data, further functional analysis should be done to elucidate the potential role of these candidate tumor suppressor genes in oral carcinogenesis.