Biological analysis of a candidate stem cell - KUSA/A1 cell - for bone tissue engineering

ANDREA P. RODRIGUEZ; HIDETSUGU TSUJIGIWA; HITOSHI NAGATSUKA; SILVIA S. BORKOSKY; TOHRU TAKAGI; HAILONG HU; RYO TAMAMURA; NORIYUKI NAGAI

Shiojiri

Congreso; The 16th Annual Meeting of the Society for Hard Tissue Regenerative Biology; 2007

Matsumoto Dental University

KUSA/A1 cell is a bone marrow stromal cell, capable of differentiating into osteoblasts under inducing conditions. We demonstrated that KUSA/A1 cells with non-osteogenic potential seeded in diffusion chamber differentiated into osteoblasts and produced mineralized bone-like tissue. Finally, we evaluated the effect of honeycomb scaffold to produce abundant bone formation using KUSA/A1 cells implanted in subcutaneous tissues of SCID mice. 1 x 106 KUSA/A1 cells with honeycomb scaffold showed abundant new bone formation. While, 5 x 106 KUSA/A1 cells alone showed only few small islands of new bone. This study supports that KUSA/A1 cell is a good candidate for basic research in bone tissue engineering.