INVESTIGADORES
ATTALLAH Carolina Veronica
congresos y reuniones científicas
Título:
Copper homeostasis in plants: role of two Arabidopsis metal chaperones involved in COX biogenesis.
Autor/es:
WELCHEN, E.; ATTALLAH, C.V.; GONZÁLEZ, D.H.
Lugar:
San Miguel de Tucumán, Tucumán. Argentina.
Reunión:
Congreso; XLV Reunión anual de la Sociedad Argentina de Investigación en Bioquímica y Biología Molecular (SAIB); 2009
Resumen:
In higher plants, copper (Cu) plays key roles in the photosynthetic
and respiratory electron transport chains and is involved in crucial
processes including ethylene perception, cell wall metabolism and
oxidative stress protection. Cu deficiency induces plant chlorosis,
mostly affecting young leaves and reproductive organs. We studied
two Arabidopis proteins, AtCOX19 (At1g66590) and AtCOX17-1
(At1g53030), putative homologues of yeast metal chaperones
involved in Cu delivery for cytochrome c oxidase (COX)
biogenesis. Arabidospsis plants overexpressing AtCOX19 and
(At1g53030), putative homologues of yeast metal chaperones
involved in Cu delivery for cytochrome c oxidase (COX)
biogenesis. Arabidospsis plants overexpressing AtCOX19 and
(At1g53030), putative homologues of yeast metal chaperones
involved in Cu delivery for cytochrome c oxidase (COX)
biogenesis. Arabidospsis plants overexpressing AtCOX19 and
Arabidopis proteins, AtCOX19 (At1g66590) and AtCOX17-1
(At1g53030), putative homologues of yeast metal chaperones
involved in Cu delivery for cytochrome c oxidase (COX)
biogenesis. Arabidospsis plants overexpressing AtCOX19 andArabidospsis plants overexpressing AtCOX19 and
AtCOX17-1 show a Cu deficiency phenotype, with severe disorders
at the vegetative and reproductive stages, affecting normal pollen
development and fertility. Although the Cu levels in 2-week-old
seedlings measured by atomic absorption spectroscopy are
comparable to those of wild-type, plants are Cu hipersensitive and
exhibit decreased root length and reduced fresh weight, that is
partially reverted by a copper-specific chelator. At the molecular
level, overexpressing plants have higher expression levels of the
stress responsive gene AOX1a, encoding the mitochondrial
alternative oxidase, and altered expression levels of genes related
with Cu metabolism. We conclude that the metal chaperones under
study have a role in regulating cellular Cu homeostasis in addition
to their known function in COX assembly.
alternative oxidase, and altered expression levels of genes related
with Cu metabolism. We conclude that the metal chaperones under
study have a role in regulating cellular Cu homeostasis in addition
to their known function in COX assembly.
alternative oxidase, and altered expression levels of genes related
with Cu metabolism. We conclude that the metal chaperones under
study have a role in regulating cellular Cu homeostasis in addition
to their known function in COX assembly.
at the vegetative and reproductive stages, affecting normal pollen
development and fertility. Although the Cu levels in 2-week-old
seedlings measured by atomic absorption spectroscopy are
comparable to those of wild-type, plants are Cu hipersensitive and
exhibit decreased root length and reduced fresh weight, that is
partially reverted by a copper-specific chelator. At the molecular
level, overexpressing plants have higher expression levels of the
stress responsive gene AOX1a, encoding the mitochondrial
alternative oxidase, and altered expression levels of genes related
with Cu metabolism. We conclude that the metal chaperones under
study have a role in regulating cellular Cu homeostasis in addition
to their known function in COX assembly.
alternative oxidase, and altered expression levels of genes related
with Cu metabolism. We conclude that the metal chaperones under
study have a role in regulating cellular Cu homeostasis in addition
to their known function in COX assembly.
alternative oxidase, and altered expression levels of genes related
with Cu metabolism. We conclude that the metal chaperones under
study have a role in regulating cellular Cu homeostasis in addition
to their known function in COX assembly.
at the vegetative and reproductive stages, affecting normal pollen
development and fertility. Although the Cu levels in 2-week-old
seedlings measured by atomic absorption spectroscopy are
comparable to those of wild-type, plants are Cu hipersensitive and
exhibit decreased root length and reduced fresh weight, that is
partially reverted by a copper-specific chelator. At the molecular
level, overexpressing plants have higher expression levels of the
stress responsive gene AOX1a, encoding the mitochondrial
alternative oxidase, and altered expression levels of genes related
with Cu metabolism. We conclude that the metal chaperones under
study have a role in regulating cellular Cu homeostasis in addition
to their known function in COX assembly.
alternative oxidase, and altered expression levels of genes related
with Cu metabolism. We conclude that the metal chaperones under
study have a role in regulating cellular Cu homeostasis in addition
to their known function in COX assembly.
alternative oxidase, and altered expression levels of genes related
with Cu metabolism. We conclude that the metal chaperones under
study have a role in regulating cellular Cu homeostasis in addition
to their known function in COX assembly.
show a Cu deficiency phenotype, with severe disorders
at the vegetative and reproductive stages, affecting normal pollen
development and fertility. Although the Cu levels in 2-week-old
seedlings measured by atomic absorption spectroscopy are
comparable to those of wild-type, plants are Cu hipersensitive and
exhibit decreased root length and reduced fresh weight, that is
partially reverted by a copper-specific chelator. At the molecular
level, overexpressing plants have higher expression levels of the
stress responsive gene AOX1a, encoding the mitochondrial
alternative oxidase, and altered expression levels of genes related
with Cu metabolism. We conclude that the metal chaperones under
study have a role in regulating cellular Cu homeostasis in addition
to their known function in COX assembly.
alternative oxidase, and altered expression levels of genes related
with Cu metabolism. We conclude that the metal chaperones under
study have a role in regulating cellular Cu homeostasis in addition
to their known function in COX assembly.
alternative oxidase, and altered expression levels of genes related
with Cu metabolism. We conclude that the metal chaperones under
study have a role in regulating cellular Cu homeostasis in addition
to their known function in COX assembly.
AOX1a, encoding the mitochondrial
alternative oxidase, and altered expression levels of genes related
with Cu metabolism. We conclude that the metal chaperones under
study have a role in regulating cellular Cu homeostasis in addition
to their known function in COX assembly.