INVESTIGADORES
COLMAN LERNER Alejandro Ariel
congresos y reuniones científicas
Título:
ENERGY ME TABOL I SM MODULAT E S THE PHEROMONE RESPONSE GPCR PATHWAY IN THE YEAST S.cerevisiae
Autor/es:
ALICIA GRANDE; KENNEDY E; RODRIGO BALTANÁS; ALEJANDRO COLMAN LERNER
Lugar:
Argentina
Reunión:
Congreso; SAIB 46th Annual Meeting Argentine Society for Biochemistry and Molecular Biology XLVI Reunión Anual Sociedad Argentina de Investigación en Bioquímica y Biología Molecular; 2010
Institución organizadora:
Society for Biochemistry and Molecular Biology XLVI Reunión Anual Sociedad Argentina de Investigación en Bioquímica y Biología Molecular
Resumen:
Oscillatory mechanisms control aspects as diverse as seasonal
breeding, circadian rhythms and early embryogenesis. Mating
pheromone in yeast activates a conserved GPCR-MAPK cascade
pathway. In high pheromone, yeast form a mating projection
(shmoo) to fuse with a partner. If no mating occurs, cells abandon
that shmoo and search in another direction, making a new shmoo
every 100 minutes. The oscillatory mechanism underlying this
searching behavior is unknown. Here we screened, by microscopy,
the complete deletion collection of non-essential kinases and
phosphatases to find mutants that form less shmoos. We selected 29
out of the 130 strains. These mutants grouped in different classes.
Interestingly, one class included 3 enzymes that regulate PDA1, the
catalytic subunit of the pyruvate dehydrogenase complex,
suggesting that respiration might modulate shmoo formation. Given
that yeast exhibit metabolic oscillations with a period of about 50
minutes, we hypothesize that the oscillations propagate, perhaps via
ethanol levels, to the pheromone response pathway. Supporting this
idea, decoupling pheromone signaling from respiration by addition
of ethanol (the end product of yeast fermentation) or deletion of
PDA1, greatly inhibited formation of multiple shmoos. We
speculate that integration of signaling with the core metabolism
might be a general strategy to elicit complex behaviors.