Synthesis and Characterization of CaCO3-Biopolymers Hybrid Microparticles for Tissue Engineering

BOSIO VALERIA E.; CALVIGNAC B; BOURY FRANK; CASTRO GUILLERMO R

La Plata

Conferencia; HK 2010- Humboldt Kolleg, International Conference on Physics; 2011

Focus on the development of hybrid materials has been received high attention in Medicine amongst other fields for bone repair and/or replace using minimally invasive surgery (MIS). The aim of the present work is study the synthesis and characterization of novel hybrid microparticles (hMPs) based on biopolymers and calcium carbonate for potential use in bone regeneration. The hMPs were also developed, as potential carrier of specific molecules towards the damaged zone to use in cancer therapies, and/or increase the repair efficiency and help tissue differentiation. Calcium carbonate were selected as calcium source, to made the hMPs matrices who will incorporate the biopolymer-active drug complexes. Development of the hMPs were synthesize by coprecipitation method in presence of biopolymers. Doxorubicin an anticancer drug, was used as molecule model to screen 10 biopolymers. Hyaluronic acid, three types of pectins and other three types of carragenines were selected based on the interaction with the drug. Optimal synthesis conditions were determined based on stability and gelification performance of each biopolymer.  hMPs characterization were performed by chemical and physical structure analysis, morphology, size and physiological pH stability. Fluorescence microscopy was used to determine the presence of the biopolymers in the CaCO3 matrices. hMPs structural analysis were performed with FTIR and Raman spectroscopies. Physical structure was determined by XRD and hMPs morphology including the size, shape, and surface properties were examined using a SEM. In order to determine the presence of the biopolymer blended into the matrices, biopolymers derivatized with fluorescent probes were synthesized. Fluorescence microscopy revealed the presence of biopolymers incorporated to inorganic matrices and confirmed by FTIR and Raman spectroscopies. Also, the spectroscopies allowed to detect the presence of vaterite and calcite crystaline structures of CaCO3. XRD analysis showed about 70/30 % vaterite/calcite in biopolymer absence, and an increase vaterite percentage up to 95% depending on the biopolymer type. hMPs stability in physiological buffer solutions was increased in presence of the biopolymers. SEM images revealed that all types of MPs were approximately spherical in shape with a average diameter 1–5 μm. In addition, all types of MPs have shown the smooth outer surface.