INVESTIGADORES
HONORE Stella Maris
congresos y reuniones científicas
Título:
Protective effect of yacon leaves extracts against nephropathy induced by experimental diabetes in rats
Autor/es:
HONORÉ S. M.; SERRA BARCELLONA C.; GENTA S. B.; SANCHEZ S.S.
Lugar:
Lima
Reunión:
Congreso; 15th Triennial Symposium of the International Society for Tropical Root Crops; 2009
Institución organizadora:
15th Triennial Symposium of the International Society for Tropical Root Crops
Resumen:
Nephropathy
is the most common cause of morbidity and mortality in patients with
diabetes. Prevent the appearance and progression of this complication
should therefore be instituted as early as possible. The present study
was conducted to characterize the efficacy and discuss possible
underlying action mechanism of Smallanthus sonchifolius (yacon) leaf extract on diabetic rats.
Streptozotocin-diabetic
rats were orally administered with yacon leaves water extract (70 mg
dry extract/kg body weigth) once a day for 60 days. Renal function
related biochemical parameters in blood and urine were analyzed. At the
end of experimental period the kidneys were isolated for histology,
immunohistochemistry staining and RT-PCR analysis. Yacon
leaves extract significantly decreased the high blood glucose levelin
diabetic rats. Diabetic-dependent alterations in urinary albumin
excretion, creatinine clearance, kidney hypertrophy and basement
membrane thickening were attenuated by yacon extract. The intensity of
oxidative stress decreased in treated animals. These finding are
associate with a marked decrease in TGF-beta mRNA level. In addition,
the expression of other molecular markers of diabetic nephropathy such
as type IV collagen, laminin-1, fibronectin, TGF-betaand Smad2/3were
diminished in yacon-treated group in comparison with the control
diabetic group. These results suggested that yacon leaves water extract
is a protective agent against renal damage in diabetic nephropathy,
which acttion can be mediated by TGF-beta/Smads signals and scavenge
reactive oxygen species.