INVESTIGADORES
HONORE Stella Maris
congresos y reuniones científicas
Título:
Endogenous BMP signalling in normal and diabetic mice
Autor/es:
HONORÉ S. M.; GENTA S. B.; SÁNCHEZ S. S.
Lugar:
Los Cocos. Córdoba
Reunión:
Workshop; 1st International Workshop: New Concepts in Developmental Biology; 2006
Institución organizadora:
Sociedad Argentina de Biologia del Desarrollo
Resumen:
The
enteric nervous system (ENS) is derived from neural crest cells that
migrate along the gastrointestinal tract to form a network of neurons
and glia that are essential for regulating intestinal motility.
Despite the number of genes known to play essential roles in ENS
development, the participation in adult tissues and under pathologica1
states remains largely unknown. Gastrointestinal disorders are common
complications in diabetic state resulting in a loss of enteric
neurons and subsequent motility alteration. The molecular etiology of
this process is not known yet. BMPs act early in gut morphogenesis
particularly regulating specification and differentiation of ENS.
In
this study, we have investigated the presence of BMP signaling
pathway in normal adult gut and their possible involvement in
gastrointestinal dysfunction in experimental model of diabetes. Under
our experimental conditions we observed alterations in the expression
of Vasoactive intestinal polypeptide and Substance P mRNAs two
neuronal transmitters implicated in smooth muscle contraction.
We showed that BMP4 expression is active in the submucosa and in the
ENS of normal intestine but is more widespread in diabetic gut
Furthermore, the mRNA profiles for BMP ligands, receptors and
cytoplasmic mediators were significantly altered during diabetes. We
observed change in levels of BMP 2 and BMP4 mRNA, in contrast any
variation was shown for BMP6 mRNA. An increase of BMP type I, II
receptors and Smadl expression was also reported. Collectively, our
data demonstrate for the first time active BMP/Smad signaling in adult
gut. Moreover, diabetic gut shows a deregulation of this signaling
pathway. Thus raises the possibility that BMPs could play a
determining role in the pathophysiology of diabetic gut.