90. Global Metabolic Response in the Bile of Horned Frog (Ceratophrys cranwelli) Tadpoles Exposed to Endosulfan

MARINO D.; SY, VICTORIA; NATALE, G.S.; CARRIQUIRIBORDE P.

Boston, MAsachussetts

Congreso; SETAC North America 32nd Annual Meeting, Boston; 2011

SETAC North America

e global metabolic response in the bile of horned frog (Ceratophrys cranwelli) tadpoles exposed to endosulfan was assessed using UPLC-MS/MS. Tadpoles were exposed to 0, 5 and 10 μg/L of endosulfan (a.i.) during 96 h. Treatments were performed by triplicate exposing seven organisms per replica. e gallbladders from ve tadpoles per replica were pooled in a microtube with 1 ml of nanopure water, centrifuged and ltered through 0.22 μm. Samples were analyzed using an UPLC system (ACQUITY UPLC®) with a C18 column (ACQUITY BEH) and couple to a tandem quadrupole mass spectrometer (Quattro Premier™ XE) equipped with an electrospray atmospheric pressure ionization interface (ZSpray™). Data were acquired using the MassLynx™ software and processed for peak detection, matching and alignment using the XCMS open access software. e highest number of ions was achieved in ESI negative mode (1122 peaks). e number of ions 10-fold up and down regulated in tadpoles exposed to 5 μg/L, respect to the controls, was 64 and 59, respectively. In tadpoles exposed to 10 μg/L the number of peaks was 90 and 57, respectively. Most of these ions belong to the same time clusters, suggesting they were fragments of the same metabolites. e strongest down regulation was observed for a peak at m/z 367, showing a clear concentration dependent response with 7,684 and 15,311 fold reduction at 5 and 10 μg/L, respectively. e highest up regulation was observed for a peak at m/z 803, showing a concentration dependent response with a mean abundance of 0, 71 and 10,843 in 0, 5, and 10 μg/L, respectively. A characteristic response at the global metabolic prole was observed in the bile of C. cranwelli exposed to endosulfan, which could be useful as ngerprint of the exposure to this organochlorine. In addition, further identication of major responding compounds could help to gain insight on the toxic mechanisms of the insecticide.