New colorimetric method for analysis of alkylresorcinols based on the use of Fast Blue RR Salt

SAMPIETRO, D. A.; VATTUONE M.A.; CATALAN, C. A. N.

Tafi del Valle (Tucuman)

Congreso; XXVI Annual Scientific Meeting of Tucuman Biology Association; 2009

Tucuman Biology Association

Intake of whole grains offers health benefits for people with chronic diseases. Whole grain foods can be detected through analysis of alkylresorcinols (ARs). However, new methods are required for fast and inexpensive analysis of these compounds. The aim of this work was to develop a new method for analysis of ARs based in the use of Fast Blue RR (FB RR) salt. The influence of different concentrations of FBRR salt, organic solvents and basifying reagents was assayed at several temperatures and light conditions. Olivetol and orcinol were used as standards. Good linearity was observed for olivetol in the range of 1–10 µg with methanol as solvent (lambda max = 480 nm) and 1–7 µg with butanol as solvent (lambda max = 530 nm). Sensitivity obtained in butanol was comparable to that obtained in the Fast Blue B based method (methanol as solvent, kmax = 520 nm). In the new colorimetric method described here, incubation time was reduced to 20 min and the stability of the reaction products was as long as 3 h. The method appears promising for the analysis of 1,3-dihydroxybenzene derivatives in samples from plant breeding and food analyses.