Aging disrupts clock and epigenetic factors circadian rhythms and makes rhythmic the clock-controlled expression of DNA repair enzymes. Effects of a caloric restricted diet

CASTRO PASCUAL, IVANNA CARLA; DAS NEVES OLIVEIRA, ANGELA; VAN HELVOORT LENGERT, ANDRE; CARGNELUTTI, ETHELINA; LACOSTE, MARÍA GABRIELA; FERRAMOLA, MARIANA LUCILA; DELGADO, SILVIA MARCELA; MELÉNDEZ, MATÍAS; ANZULOVICH, ANA CECILIA

San Luis

Congreso; XXXVIII Reunión Anual de la Sociedad Argentina de Investigación en Neurociencias; 2023

Sociedad Argentina de Investigación en Neurosciencias

Disruption of circadian rhythms and alterations in the DNA repair systems, constitute part of the biological and molecular basis of motor and cognitive aging (Lacoste et al., 2017; Langie et al., 2017). Azis Sancar et al. (2015) reported that the DNA nucleotide excision repair is regulated by the cellular clock. Given cerebellum is very susceptible to oxidative stress and DNA damage, we analyzed the aging consequences on the circadian regulation of the DNA base excision repair (BER) system and the daily profiles of BER-related epigenetic factors, in the cerebellum. We also evaluated the effect of caloric restriction (CR) and investigated the mechanism of Ogg1 and Ape1 circadian regulation. Three- and 22-mo-old rats treated or not with a 40% CR diet and maintained under constant darkness, were used in this study. We observed that BMAL1 protein, as well as Sirt1 and Dnmt1 expression display circadian rhythms(p