Modulation of C-type lectin receptors in dendritic cells by hydatid fluid from Echinococcus granulosus induces autophagy and polyfunctional T cell responses.

CHOP, MAIA; LOOS, JULIA A.; NICOLAO, MARÍA CELESTE; LEDO, CAMILA; CUMINO, ANDREA C.; RODRIGUEZ RODRIGUES, CHRISTIAN

Mar del Plata

Congreso; Reunión conjunta SAIC, SAB, AAFE, AACYTAL; 2023

SAIC, SAB, AAFE, AACYTAL

Background: Echinococcosis is caused by Echinococcus granulosus, which develops cysts filled with hydatid fluid (HF). C-type lectin receptors (CLRs) recognize carbohydrate structures leading tophagocytosis, autophagy and cytokine production. Autophagy plays a key role in antigen presentation by DCs and promotes T-cell activation. The aim of this study was to analyze whether HF can be recognized by CLRs and induce autophagy to polarize the T cell response in vitro. Methods: HF was collected from infected cattle. Autophagy induction in BMDCs was evaluated by FACS, qPCR and confocal microscopy. The expression of CLRs were evaluated by FACS. Cytokine profiles were analyzed by qPCR. Results: HF-stimulated BMDCs significantly enhanced the number (**p