PROTEIN KINASE D1 ACTIVITY IS ASSOCIATED TO MOUSE SPERM CAPACITATION

EDUARDO MARTÍNEZ LEÓN; OSYCKA-SALUT, CLAUDIA; MARIN-BRIGGILER, CL; JABLOÑSKI, M.; GÓMEZ, MATÍAS; BUFFONE, MARIANO; REY, OSVALDO

Mar del Plata

Congreso; Reunión Anual de Sociedades de Biociencias; 2023

SAIC-SAB-AAFE-AACYTAL

Sperm are specialized and transcriptionally inactivecells dependent on post-translational modifications, such as proteinphosphorylation, to carry out their functions. These changes occur during theprocess called sperm capacitation. The protein kinase D family (PKDs) includesthree serine/threonine kinases (PKD1, PKD2 and PKD3) being PKD1 the moststudied and ubiquitous one. This family of kinases are involved in fundamentalbiological process including signal transduction, cytoskeleton remodeling, golgitransport and oxidative stress among other functions. Recent results from our laboratoryindicate that PKD1 is present in the sperm of human, mice, equine and bovine. Sincethere are no previous reports about the presence and function(s) of any PKD inmammal sperm, we examined its distribution and function(s)    .We observed that: 1) PKD1 is present and catalyticallyactive (p-PKD) in bovine, equine, mice, and human sperm (IIF/WB). 2) The capacitation processinduces changes in kinase localization and activation levels, evaluated bysuper resolution microscopy in mouse sperm.  3) Mouse sperm incubated inthe presence of specific PKD inhibitors (Kb142-70 and CRT0066101) before spermatozoacapacitation enhanced tyrosine-phosphorylation and phospho-PKA substratesproteins levels (p