ANTIHYPERGLYCAEMIC ACTIVITY OF TWO SPECIES OF PHYLLANTHUS

GONZALEZ MIRAGLIOTTA ANA; OJEDA GONZALO ADRIAN; TORRES ANA MARÍA; NELIDA MARIA PERUCHENA

Mar del Plata

Congreso; LV Reunión Anual de de la Asociacion Argentina de Farmacologia Experimental (2023); 2023

Asociación Argentina de Farmacologia Experimental (AAFE)

Phyllanthus niruri L. (rompepiedra) and P. sellowianus (sarandí blanco), are native species of Central and South America. In ethnopharmacology, P. niruri is used as a diuretic, while P. sellowianus is used as a hypoglycemic. The aim of the works was to evaluate the inhibitory potential of these extracts on α-glucosidase enzymes (APG) from different sources. Aerial Parts (APn) and Roots (Rn) of P. niruri collected in Corrientes-Capital, along with Leaves (Ls), Stem Bark (SBs), and Roots (Rs) of P. sellowianus from Corrientes-Monte Caseros, were employed for this research. The extracts were prepared with methanol: ethyl acetate (7:3) and dried using a rotary evaporator. Antihyperglycemic efficacy was studied based on the ability of the extracts to inhibit APG activity. Results were quantified in terms of inhibition ratios (Ir), with acarbose as positive control. Additionally, phytochemistry and the HPLC-DAD profiles of the extracts were evaluated. In the Saccharomyces cerevisiae APG inhibition assay, extracts from both species outperformed the acarbose. The most active extracts were SBs: Ir=318.69, Ls: Ir=277.93 and APn: Ir=251.67. However, during the pig APG inhibition assay, all extracts exhibited lower potency (Ir