Inulin reverts intestinal multidrug resistance associated protein 2 down-regulation in high-fat diet-induced obese mice

ZECCHINATI, FELIPE; RICARDI, LAURA; BARRANCO, MARÍA MANUELA; PERDOMO, VIRGINIA GABRIELA; MOTTINO, ALDO DOMINGO; GARCÍA, FABIANA; VILLANUEVA, SILVINA STELLA MARIS

Buenos Aires

Congreso; SOCIEDAD ARGENTINA DE FISIOLOGÍA (SAFIS) 2023; 2023

Introduction. Intestinal multidrug resistance associated protein 2 (Mrp2) is an ABC transporter that limits the absorption of xenobiotics and drugs orally ingested, thus acting as a biochemical barrier. This function may be disrupted under pathophysiological conditions such as obesity, where the intestinal microbiota is altered. In previous studies we observed that standard diet enriched with 40% kcal (HFD) for 8 weeks, reduced the expression and activity of intestinal Mrp2 in mice.Objectives. Thus, we here evaluated the potential effect of inulin administration (5 % w/w), a prebiotic well-known gut modulator, in reverting HFD induced Mrp2 alterations. Methods. Proximal jejunum from C57BL/6 male mice was removed to study Mrp2 expression by western blot, and its transport activity, by using the everted intestinal sacs model. Results. After obese-like conditions were evoked, animals cotreated with inulin for another 2 weeks, showed a marked decrease in plasma triglycerides and glycemia levels, as well as an improvement in glucose homeostasis and fat deposition. Prebiotic supplementation was able to revert downregulation of Mrp2 protein expression (-57%, p