cBiMPs is a cAMP analog that potently enhances human sperm motility

OSCOZ SUSINO N; MINOTTI MF; OTERO P; KRAPF D; BUFFONE MG; MARIN BRIGGILER, CI

Congreso; Reunión Anual de Sociedades de Biociencias; 2023

cBIMPS IS A cAMP ANALOG THAT POTENTLY ENHANCES HUMAN SPERM MOTILITY.Natalia Oscoz-Susino1, Florencia Minotti2, Patricia Otero2, Dario Krapf3, Mariano G. Buffone1, Clara I. Marín Briggiler1. 1IBYME-CONICET, Buenos Aires, Argentina. 2 Hospital G. A. Carlos G. Durand, Buenos Aires, Argentina. 3IBR-CONICET, Rosario, Argentina. Cyclic AMP (cAMP) orchestrates different aspects of sperm function that are necessary for the acquisition of fertilizing capacity. It has a role in the development of motility and hyperactivation (HA) and in the occurrence of acrosomal exocytosis (AE) by the activation of a protein kinase (PKA), leading to protein phosphorylation on tyrosine residues (pY). Such responses can be mimicked by cAMP analogs, which have been used in the management of sperm in vitro. The objective of our study is to analyze the effect of a new cAMP analogue, Sp-5,6-DCl-cBIMPs (cBIMPs), on human sperm functional parameters. Motile sperm from normozoospermic men were incubated for up to 4 h with cBIMPs (0.01, 0.03 and 0.1 mM) or DMSO (0.2 %) as a control. Motility parameters were evaluated by a CASA system (SCA, Microptic), protein phosphorylations in PKA substrates (pPKA) and pY by Western blot, AE by Pisum sativum agglutining staining and DNA fragmentation by the TUNEL assay. The results showed a significant increase in % total and progressive motility, in all kinematic parameters and in % HA for all cBIMPs concentrations in comparison to the control (n=6; p