Isolation and characterization of an abietane diterpene from Coleus neochilus with potent antiproliferative activity on a human breast cancer cell line

MAYORA JUSTEL CARLA LUCIANA; VALLADARES TAMARA; CAVALLARO VALERIA; MURRAY ANA PAULA; LÜTHY ISABEL ALICIA; ESANDI MARÍA DEL CARMEN; BRUZZONE ARIANA; ALZA NATALIA

Mar del Plata

Congreso; Reunión Anual de Sociedades de Biociencias 2023 (SAIC-SAB-AAFE-AACyTAL); 2023

SAIC-SAB-AAFE-AACyTAL

ISOLATION AND CHARACTERIZATION OF AN ABIETANE DITERPENE FROM COLEUS NEOCHILUS WITH POTENT ANTIPROLIFERATIVE ACTIVITY ON A HUMAN BREAST CANCER CELL LINECarla Luciana Mayora Justel1,2, Tamara Valladares1, Valeria Cavallaro3,4, Ana Paula Murray3,4, Isabel Alicia Lüthy5, María del Carmen Esandi1,2, Ariana Bruzzone1, Natalia Alza1,41Instituto de Investigaciones Bioquímicas Bahía Blanca-CONICET, 2 Departamento de Biología Bioquímica y Farmacia - UNS, 3 INQUISUR-CONICET, 4 Departamento de Química (UNS), 5 Instituto de Biología y Medicina Experimental-CONICETColeus spp. have diverse ethnobotanical applications, with their most prevalent use being attributed to their medicinal properties. We have previously described the antiproliferative effect of the ethanolic extract from C. neochilus (also known as “boldo rastrero”) on different human breast cancer cell lines. The present study is focused on the extraction and isolation of compounds responsible for this effect. The ethanol extract (BRET) was subjected to solvent-solvent extraction, yielding four different sub-extracts: hexane (BRHX), chloroform (BRCL), ethyl acetate (BRAE), and methanol-water (BRAM). Remarkably, incubation with BRAE (50 μg/ml) was more effective in reducing the viability of human breast cancer cell line MCF-7 than the other fractions (BRET: 23%, BRHX: 29%, BRCL: 52%, BRAE: 68%, and BRAM: 10% reduction; p