ADENYLATE CYCLASE ISOENZYMES PARTICIPATE IN HYALURONIC ACID CAPACITATION OF BOVINE SPERMATOZOA

SILVINA FERNÁNDEZ; SERGIO MORADO; PABLO CETICA; MARIANA CÓRDOBA

Congreso; Reunión Conjunta de Sociedades Biomédicas; 2023

The aim of this study was to evaluate the effect of adenylate cyclase isoenzymes inhibition in bovine sperm functional parameters, such as capacitation, motility, viability/acrosomal integrity, mitochondrial activity and in vitro fertilization (IVF). Hyaluronic acid (HA) was used as a sperm capacitation inducer, LRE-1 as soluble adenylate cyclase inhibitor, and 2,5-dideoxyadenosine (2,5-D) as membrane adenylate cyclase inhibitor. Five treatments were performed with frozen-thawed semen in TALP medium at 38°C: control, HA, HA/LRE-1, HA/2,5-D, and HA/LRE-1/2,5-D. Sperm capacitation was evaluated by the chlorotetracycline epifluorescent technique and viability/acrosomal integrity by trypan blue vital staining with differential interferential contrast. Sperm motility was evaluated by microscopy and analyzed with the ISAS-Prosier software. Mitochondrial membrane potential of spermatozoa was measured using the fluorochrome JC-1. Cleavage rate was analyzed 48 hours after IVF. Data were analyzed by ANOVA and Tukey's test (P